Journal: PLoS ONE

Article Title: Long Non-Coding RNA Expression Profiling of Mouse Testis during Postnatal Development

doi: 10.1371/journal.pone.0075750

Figure Lengend Snippet: Summary of microarray analysis results.

Article Snippet: Labeled cDNA was hybridized to the Mouse Stringent LncRNA Microarray (Arraystar) using Agilent’s SureHyb Hybridization Chambers according to the One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology).

Techniques: Microarray

Journal: PLoS ONE

Article Title: Long Non-Coding RNA Expression Profiling of Mouse Testis during Postnatal Development

doi: 10.1371/journal.pone.0075750

Figure Lengend Snippet: The vertical axis indicates relative expression levels of each lncRNA. The relative expression levels were assessed by Q-PCR and were normalized with GAPDH gene. Each result was the average of three independent biological replicates. The horizontal axis indicates the six time points of postnatal testis development from 6 days (d) to 8 weeks (w) after birth.

Article Snippet: Labeled cDNA was hybridized to the Mouse Stringent LncRNA Microarray (Arraystar) using Agilent’s SureHyb Hybridization Chambers according to the One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Long Non-Coding RNA Expression Profiling of Mouse Testis during Postnatal Development

doi: 10.1371/journal.pone.0075750

Figure Lengend Snippet: Validation of microarray results by qRT-PCR.

Article Snippet: Labeled cDNA was hybridized to the Mouse Stringent LncRNA Microarray (Arraystar) using Agilent’s SureHyb Hybridization Chambers according to the One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology).

Techniques: Biomarker Discovery, Microarray

Journal: PLoS ONE

Article Title: Long Non-Coding RNA Expression Profiling of Mouse Testis during Postnatal Development

doi: 10.1371/journal.pone.0075750

Figure Lengend Snippet: Examples of exonic sense (A), exonic antisense (B), intronic sense (C), intronic antisense (D), bidirectional (E and F), and intergenic (G) lncRNAs are shown. Each panel illustrates the organization of the lncRNA (red) to associated protein-coding genes (blue). CpG islands are indicated by green strips. Arrows indicate the direction of transcription.

Article Snippet: Labeled cDNA was hybridized to the Mouse Stringent LncRNA Microarray (Arraystar) using Agilent’s SureHyb Hybridization Chambers according to the One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology).

Techniques:

Journal: PLoS ONE

Article Title: Long Non-Coding RNA Expression Profiling of Mouse Testis during Postnatal Development

doi: 10.1371/journal.pone.0075750

Figure Lengend Snippet: Each panel illustrates the lncRNA (red) transcription initiation direction (indicated by an arrow) relative to an annotated microRNA (green). (A) H19 overlaps with mmu-mir-675. (B) AK144366 overlaps with mmu-mir-202. (C) Tissue expression pattern of AK144366 , according to the Affymetrix Exon Tissues Track in the UCSC Genome Browser. Red indicates high level of expression.

Article Snippet: Labeled cDNA was hybridized to the Mouse Stringent LncRNA Microarray (Arraystar) using Agilent’s SureHyb Hybridization Chambers according to the One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology).

Techniques: Expressing